New DNA Extraction System

A new DNA extraction system is easier, due to fewer steps, and takes less time than standard spin-column extraction methods.

The MycoExtract buffer provides for the extracted DNA to be precipitated and re-suspended in water. Further, the extracted DNA can be used directly for molecular procedures such as polymerase chain reaction (PCR), which results in a further saving of time. According to a bioinformatics study, nearly 84% of scientists surveyed use PCR when extracting DNA. Also, because lysis in the new extraction procedure is accomplished by a 30-minute incubation period at 95oC, any unlysed cells are killed and the final steps of the procedure--as well as any downstream procedures-- can be done outside of a biocontainment hood.

Initial applications of molecular diagnostics were mostly for infections but are now increasing in the areas of genetic disorders, preimplantation screening, and cancer, according to NanoLogix, Inc. (Sharon, PA, USA), which developed the new DNA extraction system. Genetic screening tests are a promising area for future expansion of the in vitro diagnostics market, the company notes. Molecular diagnostics are being combined with therapeutics, and molecular diagnostic technologies are also involved in the development of personalized medicine.

"The NanoLogix buffer allowed the DNA extraction from different strains of mycobacteria, requiring a single buffer, one incubation, and one post-lysis centrifugation, compared to other products,” reported Paul Hyman, Ph.D., a researcher at Ohio State University (Columbus, OH, USA).




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