Proteins Profiled on Antibody Arrays

Universal linkage system (ULS) protein labeling and fluorescent detection kits have been developed for profiling serum and cell lysate samples on high-content antibody arrays.

The four single-color ULS protein labeling kits are an addition to the two-color protein labeling kits already available from Kreatech Biotechnology (Amsterdam, The Netherlands). The ULS labeling molecule is bound to biotin or fluorescein for direct labeling of protein analytes in serum and cell lysate samples. The kits have been validated on currently available antibody array platforms.

The complete kits include the appropriate blocking and washing buffers as well as the unique purification column, USL-Trap, which ensure the complete removal of unreacted dye while yielding a high sample recovery that is independent of protein size. Upon incubation of the antibody array, detection is provided by anti-fluorescein-DY 647 or streptavidin-DY 647 conjugates, allowing scanning of the array in the standard CyTM5 channel. The ULS acts as a relatively simple "molecular glue” for proteins, DNA, or RNA.

Kreatech's technology relies on the special binding properties of transition metals, especially platinum, to form coordinative bonds with biomolecules by binding to specific sites on DNA, RNA, and proteins. The molecular label attached to the ULS can be chosen according to the biomolecules of interest.

In addition to kits for protein labeling, Kreatech also provides labeling kits for antisense RNA (aRNA) labeling, small RNA/microRNA labeling, array comparative genomic hybridization (CGH) labeling, and general nucleic acid labeling.


Related Links:
Kreatech Biotechnology