Novel Technology Halves Time for Real-Time PCR

A novel technology significantly reduces processing times of the real-time polymerase chain reaction (PCR). It can be used on all real-time instruments.

Quantifast technology is based on fast-cycling PCR. Kits allow customers to reduce the duration of PCR to 40–60 minutes. Users can save more than 50% of the time previously needed, without compromising sensitivity or reproducibility.

The PCR is a one of the most widely used and important technologies in modern molecular biology, which allows the detection of genetic targets in samples. Real-time PCR expands the information available in PCR assays by providing online, real-time quantification of the target genetic material.

Qiagen (Hilden, Germany) has launched the Quantifast line, which significantly increases speed in real-time PCR assays. The kits are compatible with all commercially available real-time PCR instruments, known as thermocyclers, and with existing PCR assays. They can be used with these without requiring complex adaptations (such as new primer design or optimization of PCR protocols). The Quantifast kits enable higher sample throughput using existing laboratory apparatus or established real-time PCR systems. This avoids the need for expensive purchases of faster PCR instruments.

"The unique Quantifast kits broaden Qiagen's already leading portfolio of assay technologies for research, applied testing, and molecular diagnostics and are further evidence of Qiagen's technology leadership”, said Dr. Janina Lehmann, global business director, gene expression at Qiagen. "The innovative Quantifast kits enable users to more than double their throughput without compromising quality, cost, and assay design. These advantages allow our customers fast and immediate access to a new dimension of possibilities in real-time PCR.”

The Quantifast kits can be used for screenings in applied testing, to deliver reliable diagnostic results more rapidly in acute epidemics as a component of a self-validated test system, and to speed up drug development in the pharmaceutical industry.



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